RESUMEN
Worldwide, equines are affected by equine filariosis, an endemic vector-borne disease caused by heterogenous parasitic nematodes. The main objective of this study was to determine the prevalence of filarial infection among horses in the North and Northeast of Iran. Between October 2021 and July 2022, 145 equine blood samples were investigated, of which 49 cases were from the northeast and 96 were from the north of Iran. Blood samples were taken for microfilariae using Knott's method. Out of 145 blood samples, only 2 cases (1.37%) from northern Iran were positive for microfilaria of Setaria equina, and no positive samples were found from northeast Iran. The first positive sample was a 3-year-old male Iranian horse displaying clinical symptoms of weakness, while the second, an 8-month-old Arabian breed colt, exhibited no clinical signs. According to the results of the current study, the prevalence of equine filariosis is low, contrary to nearby countries. To prevent and control filarial infections among horses and improve their management system, it is therefore important to emphasize filarial infection prevention, control, and management.
RESUMEN
BACKGROUND: Parasitic infections pose significant threats to humans' and animals' well-being worldwide. Among these parasites, Halicephalobus spp., a genus of nematodes, has gained attention due to its ability to cause severe infections in various animal species, including horses. OBJECTIVE: This study aimed to determine the prevalence of Halicephalobus spp., specifically focusing on Halicephalobus gingivalis in horses. MATERIALS AND METHODS: In July 2022, a cross-sectional study was conducted in northern Iran to determine the prevalence of Halicephalobus spp. Using standard coprological techniques, 141 fecal samples from randomly selected horses were analyzed for GI helminth eggs. The Halicephalobus spp. eggs present in faeces were identified by molecular methods. Polymerase Chain Reaction (PCR) was used to amplify the partial 5' variable region (~ 390 base pairs) of 18 S DNA using SSUA_F and SSU22_R primers. Furthermore, the PCR products obtained were sequenced, and phylogenetic analysis was performed using available sequences from GenBank. RESULTS: Microscopic examination of 141 fresh faecal samples revealed 5 fecal samples were infected with small ellipsoidal nematode eggs ranging between 40 and 50 × 50-60 µm. This study's PCR amplicons showed ~ 390 bp bands on 2.0% agarose gel. A partial sequence of 18 S DNA (363 bp) was obtained herein (GenBank accession no. OQ843456). CONCLUSION: Overall, using molecular tools represents a significant step forward in diagnosing and managing the Halicephalobus gingivalis infections in horses.
